Abstract
Radiation exposure is particularly damaging to cells of the hematopoietic system, inducing pancytopenia and bone marrow failure. The study of these processes, as well as the development of treatments to prevent hematopoietic damage or enhance recovery after radiation exposure, often require analysis of bone marrow cells early after irradiation. While flow cytometry methods are well characterized for identification and analysis of bone marrow populations in the nonirradiated setting, multiple complications arise when dealing with irradiated tissues. Among these complications is a radiation-induced loss of c-Kit, a central marker for conventional gating of primitive hematopoietic populations in mice. These include hematopoietic stem cells (HSCs), which are central to blood reconstitution and life-long bone marrow function, and are important targets of analysis in these studies. This chapter outlines techniques for HSC identification and analysis from mouse bone marrow postirradiation.
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Acknowledgments
This work was supported by DOD partnering grant W1XWH-15-1- 0254/0255, NIH grant HL096305, and NIH T32 training grant HL007910. Flow cytometry was conducted in the Indiana University Melvin and Bren Simon Comprehensive Cancer Center (IUSCCC) Flow Cytometry Resource Facility (FCRF), funded in part by NCI grant P30 CA082709, NIDDK grant U54 DK106846, NIH instrumentation grant 1S10D012270, and the Center of Excellence Grant in Molecular Hematology grant PO1 DK090948.
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Patterson, A.M., Orschell, C.M., Pelus, L.M. (2023). Hematopoietic Stem Cell Identification Postirradiation. In: Pelus, L.M., Hoggatt, J. (eds) Hematopoietic Stem Cells. Methods in Molecular Biology, vol 2567. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2679-5_9
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DOI: https://doi.org/10.1007/978-1-0716-2679-5_9
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