Abstract
Fecal pollution in surface waters is a major threat to recreational and drinking water resources, with Escherichia coli being a primary concern. The best way to mitigate fecal pollutant loading is to identify the sources and tailor remediation strategies to reduce loading. Tracking E. coli back to its source is notoriously difficult in a mixed-use watershed where input from humans, wildlife, and livestock all contribute to E. coli loading. One proposed tracking method for E. coli contamination is the use of fecal sterols and sterol ratios. This study uses fecal sterol data published globally to assess how well sterol compositions for different species clusters along with the effectiveness of sterol ratios as tracking tools. Hierarchical cluster analysis produces stronger clusters based on sterol ratios than raw sterol concentration, but the global dataset results in clustering of the same species in different levels. The accuracy of the sterol ratios was also compared to understand the rate of false negatives and false positive assignments. Overall, these ratios did not have a high success rate for determining the correct source, which was also reflected in the poor clustering trends observed. Establishing local end-member sterol profiles is essential when using sterol signatures to unravel fecal loading.
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References
Bull ID, Lockheart MJ, Elhmmali MM et al (2002) The origin of faeces by means of biomarker detection. Environ Int 27:647–654. https://doi.org/10.1016/s0160-4120(01)00124-6
de Jong A, Plat J, Mensink RP (2003) Metabolic effects of plant sterols and stanols (review). J Nutr Biochem 14:362–369. https://doi.org/10.1016/s0955-2863(03)00002-0
Devane ML, Moriarty EM, Robson B et al (2019) Relationships between chemical and microbial faecal source tracking markers in urban river water and sediments during and post-discharge of human sewage. Sci Total Environ 651:1588–1604. https://doi.org/10.1016/j.scitotenv.2018.09.258
Devane ML, Wood D, Chappell A et al (2015) Identifying avian sources of faecal contamination using sterol analysis. Environ Monit Assess. https://doi.org/10.1007/s10661-015-4800-3
Edberg SC, Rice EW, Karlin RJ, Allen MJ (2000) Escherichia coli: the best biological drinking water indicator for public health protection. J Appl Microbiol. https://doi.org/10.1111/j.1365-2672.2000.tb05338.x
Eyssen H (1973) Role of the gut microflora in metabolism of lipids and sterols. Proc Nutr Soc 32:59–63. https://doi.org/10.1079/pns19730016
Fattore E, Benfenati E, Marelli R et al (1996) Sterols in sediment samples from Venice Lagoon, Italy. Chemosphere 33:2383–2393. https://doi.org/10.1016/s0045-6535(96)00340-2
Furtula V, Liu J, Chambers P et al (2011) Sewage treatment plants efficiencies in removal of sterols and sterol ratios as indicators of fecal contamination sources. Water Air Soil Pollut 223:1017–1031. https://doi.org/10.1007/s11270-011-0920-8
Glassmeyer ST, Furlong ET, Kolpin DW et al (2005) Transport of chemical and microbial compounds from known wastewater discharges: potential for use as indicators of human fecal contamination. Environ Sci Technol 39:5157–5169. https://doi.org/10.1021/es048120k
Grimalt JO, Fernandez P, Bayona JM, Albaiges J (1990) Assessment of fecal sterols and ketones as indicators of urban sewage inputs to coastal waters. Environ Sci Technol 24:357–363. https://doi.org/10.1021/es00073a011
Isobe KO, Tarao M, Zakaria MP et al (2002) Quantitative application of fecal sterols using gas chromatography−mass spectrometry to investigate fecal pollution in tropical waters: Western Malaysia and Mekong Delta, Vietnam. Environ Sci Technol 36:4497–4507. https://doi.org/10.1021/es020556h
Jang J, Hur H-G, Sadowsky MJ et al (2017) Environmental Escherichia coli: ecology and public health implications-a review. J Appl Microbiol 123:570–581. https://doi.org/10.1111/jam.13468
Leeming R, Ball A, Ashbolt N, Nichols P (1996) Using faecal sterols from humans and animals to distinguish faecal pollution in receiving waters. Water Res 30:2893–2900. https://doi.org/10.1016/s0043-1354(96)00011-5
Leeming R, Latham V, Rayner M, Nichols P (1997) Detecting and distinguishing sources of sewage pollution in Australian inland and coastal waters and sediments. ACS Symposium Series 306–319. https://doi.org/10.1021/bk-1997-0671.ch020
Leeming R, Nichols PD, Hewlett R, Bate N (1998) Discriminating faecal pollution: a case study of stormwater entering Port Phillip Bay, Australia. Water Sci Technol. https://doi.org/10.1016/s0273-1223(98)00728-8
Lu Y, Philp R, Biache C (2016) Assessment of fecal contamination in Oklahoma water systems through the use of sterol fingerprints. Environments 3:28. https://doi.org/10.3390/environments3040028
Naidoo S, Olaniran A (2013) Treated wastewater effluent as a source of microbial pollution of surface water resources. Int J Environ Res Public Health 11:249–270. https://doi.org/10.3390/ijerph110100249
Patton D, Reeves AD (1999) Sterol concentrations and temporal variations on the North Shore mudflats of the firth of Tay, Scotland. Mar Pollut Bull 38:613–618. https://doi.org/10.1016/s0025-326x(99)00068-5
Reeves AD, Patton D (2001) Measuring change in sterol input to estuarine sediments. Physics and Chemistry of the Earth, Part B: Hydrology, Oceans and Atmosphere 26 (9):753–757. https://doi.org/10.1016/S1464-1909(01)00081-8
Sekimoto H, Shimada O, Makanishi M et al (1983) Interrelationship between serum and fecal sterols. Jpn J Med 22:14–20. https://doi.org/10.2169/internalmedicine1962.22.14
Shah VG, Hugh Dunstan R, Geary PM et al (2007a) Bacterial source tracking from diverse land use catchments by sterol ratios. Water Res 41:3667–3674. https://doi.org/10.1016/j.watres.2007.02.050
Shah VG, Hugh Dunstan R, Geary PM et al (2007b) Evaluating potential applications of faecal sterols in distinguishing sources of faecal contamination from mixed faecal samples. Water Res 41:3691–3700. https://doi.org/10.1016/j.watres.2007.04.006
Sinton LW, Finlay RK, Hannah DJ (1998) Distinguishing human from animal faecal contamination in water: a review. NZ J Mar Freshwat Res 32:323–348. https://doi.org/10.1080/00288330.1998.9516828
Tyagi P, Edwards DR, Coyne MS (2007) Use of sterol and bile acid biomarkers to identify domesticated animal sources of fecal pollution. Water Air Soil Pollut 187:263–274. https://doi.org/10.1007/s11270-007-9514-x
Wayland D, Megson DP, Mudge SM et al (2008) Identifying the source of nutrient contamination in a lagoon system. Environ Forensics 9:231–239. https://doi.org/10.1080/15275920802122833
Writer JH, Leenheer JA, Barber LB et al (1995) Sewage contamination in the upper Mississippi River as measured by the fecal sterol, coprostanol. Water Res 29:1427–1436. https://doi.org/10.1016/0043-1354(94)00304-p
Xiao L, Estellé J, Kiilerich P et al (2016) A reference gene catalogue of the pig gut microbiome. Nat Microbiol. https://doi.org/10.1038/nmicrobiol.2016.161
Acknowledgements
We are grateful to P. Tyagi and M. Coyne for providing data from Tyagi et al. (2007) to include in this meta-analysis. We also acknowledge the reviewers and editors that provided feedback to improve the manuscript.
Funding
This work was funded in part by the Auburn University Intramural Grants Program to ASO and the Alabama Water Resources Research Institute Graduate Student Grant to EAL and ASO.
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Eleanore Larson: writing—original draft/reviewing and editing, data analysis, data curation, funding acquisition. Ayomide Afolabi: software, investigation, data analysis, writing—review and editing. Jingyi Zheng: supervision, writing—review and editing. Ann S. Ojeda: supervision, visualization, writing—review and editing, funding acquisition.
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Larson, E.A., Afolabi, A., Zheng, J. et al. Sterols and sterol ratios to trace fecal contamination: pitfalls and potential solutions. Environ Sci Pollut Res 29, 53395–53402 (2022). https://doi.org/10.1007/s11356-022-19611-2
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DOI: https://doi.org/10.1007/s11356-022-19611-2